Conjugated Dienoic Acid Peroxides as Substrates in Chaetopterus Bioluminescence System

Biochemistry of bioluminescence of the marine parchment tubeworm Chaetopterus has been in research focus for over a century; however, the results obtained by various groups contradict each other. Here, we report the isolation and structural elucidation of three compounds from Chaetomorpha linum algae, which demonstrate bioluminescence activity with Chaetopterus luciferase in the presence of Fe2+ ions. These compounds are derivatives of polyunsaturated fatty acid peroxides. We have also obtained their structural analogues and demonstrated their activity in the bioluminescence reaction, thus confirming the broad substrate specificity of the luciferase.


Introduction
Bioluminescence, or emission of light by living organisms, is widespread in nature. The process is normally caused by the oxidation of a special substrate, named luciferin, by oxygen from the air, catalyzed by an enzyme, luciferase [1]. Bioluminescence systems demonstrate amazing diversity: more than 40 different bioluminescent mechanisms are known; however, structures for only 10 luciferins are elucidated [2]. Bioluminescence is widely applied in biotechnology [3]. Investigation of the least-studied bioluminescence systems is of both fundamental and practical interest [4] ensuring the development of new methods in bioimaging, drug screening, environmental monitoring, and other applications.
Previously, we reported the characterization of the Chaetopterus variopedatus bioluminescence system components, including luciferin-luciferase activity upon the addition of exogenous ferrous ions Fe 2+ , as well as the preparation of partially purified C. variopedatus luciferase [9]. However, despite the fact that the initial extracts obtained from C. variopedatus biomass demonstrated high bioluminescent activity, the isolation of active luciferin in sufficient amounts was not possible. Heating led to a complete loss of luciferin luminescent activity; therefore, we could not obtain a "hot" extract. Solvent extracts from biomass and primary aqueous extracts also did not demonstrate significant luminescent activity of luciferin. The most efficient extract of a primary biomass homogenate would have required processing several tens of kilograms of C. variopedatus biomass to allow luciferin in amounts sufficient for LC-MS (liquid chromatography-mass-spectrometry) analysis, which was unrealistic to accomplish.
Inspired by a previous strategy used for the elucidation of fungal luciferin [10], we decided to look for precursors and analogues of C. variopedatus luciferin in other marine organisms. Substances exhibiting luciferin activity with C. variopedatus luciferase were found in a number of organisms of both plant and animal origin (soft corals, cephalopods, algae), with seaweed C. linum containing the highest amount of it. Hence, we focused on the development of a method for the isolation, purification, and elucidation of the chemical structure of active bioluminescent compounds from algae biomass.
Ethanol extraction from wet C. linum biomass followed by RP-HPLC (reversedphase high-performance liquid chromatography) allowed us to obtain three substrates, which demonstrate bioluminescent activity with luciferase-containing fraction, in sufficient amounts for further analysis. The compounds were named Hitik1, Hitik2, and Hitik3 according to the order in which they were eluted ( Figure 1, Figure S1, ).
Structural elucidation of purified compounds started with NMR (nuclear magnetic resonance) analysis of 2D DQF-COSY (double quantum filtered correlated spectroscopy), 2D 1 H-13 C HSQC (heteronuclear single quantum coherence), 2D 1 H-13 C HMBC (heteronuclear multiple bond correlation), and 2D 1 H-13 C HSQC-TOCSY (total correlation spectroscopy) spectra recorded in DMSO-d 6 (dimethyl sulfoxide). The high quality of the NMR spectra obtained allowed for straightforward elucidation, since all quaternary carbons were observed in HMBC and 1D 13 C spectra, and all labile OH protons were clearly visible in DMSO solution. First, we determined the structure of Hitik3. According to the NMR spectra, Hitik3 is a linear molecule and contains a carboxyl group (174.89 ppm and a broad proton signal at~12 ppm), two double bonds, and a hydroperoxide group (singlet proton at 11.27 ppm and ternary carbon at 85.1 ppm). The structure of Hitik3 (Figure 1) corresponds to (10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoic acid or 9-(E,Z)-HPODE. The configuration of double bonds was determined on the basis of J-coupling values, which were equal to 15 Hz for the bond 10-11 and to 11 Hz for the bond 12-13. The structure of Hitik3 is also supported by MS analysis with the mass of molecular ion M-Hequal to m/z 311.2231 and an additional peak in the MS at m/z 293.2124 (−18 Da), being the reduction product with a keto group in position 9 instead of the hydroperoxide moiety (characteristic to hydroperoxides). On the left-structures of the compounds Hitik1, Hitik2, Hitik3 (with selective atom numbering) corresponding to the chromatographic peaks 1, 2, and 3 (indicated on the inset). On the rightchromatographic profile of an ethanol extract sample on a ZORBAX Eclipse XDB-C18 column (9.4 × 250 mm). In the middle-mean luminescence activity of isolated peaks with C. variopedatus luciferase in RLU (relative light units) (measured in triplicate, expressed as mean ± SD (n = 3) and analyzed using Student's t-test; differences were considered significant at p < 0.05).
Structural elucidation of purified compounds started with NMR (nuclear magnetic resonance) analysis of 2D DQF-COSY (double quantum filtered correlated spectroscopy), 2D 1 H-13 C HSQC (heteronuclear single quantum coherence), 2D 1 H-13 C HMBC (heteronuclear multiple bond correlation), and 2D 1 H-13 C HSQC-TOCSY (total correlation spectroscopy) spectra recorded in DMSO-d6 (dimethyl sulfoxide). The high quality of the NMR spectra obtained allowed for straightforward elucidation, since all quaternary carbons were observed in HMBC and 1D 13 C spectra, and all labile OH protons were clearly visible in DMSO solution. First, we determined the structure of Hitik3. According to the NMR spectra, Hitik3 is a linear molecule and contains a carboxyl group (174.89 ppm and a broad proton signal at ~12 ppm), two double bonds, and a hydroperoxide group (singlet proton at 11.27 ppm and ternary carbon at 85.1 ppm). The structure of Hitik3 ( Figure 1) corresponds to (10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoic acid or 9-(E,Z)-HPODE. The configuration of double bonds was determined on the basis of J-coupling values, which were equal to 15 Hz for the bond 10-11 and to 11 Hz for the bond 12-13. The structure of Hitik3 is also supported by MS analysis with the mass of molecular ion M-Hequal to m/z 311.2231 and an additional peak in the MS at m/z 293.2124 (−18 Da), being the reduction product with a keto group in position 9 instead of the hydroperoxide moiety (characteristic to hydroperoxides).
Compounds Hitik1 and Hitik2 ( Figure 1) provided indistinguishable mass spectra with the major peaks at m/z 483.2596, 501.2699, and 536.3078 in positive mode. Analysis of the NMR spectra revealed that both compounds contained three identical residues with altered modes of linkages: hexopyranose carbohydrate, glycerol, and a modified polyun- On the left-structures of the compounds Hitik1, Hitik2, Hitik3 (with selective atom numbering) corresponding to the chromatographic peaks 1, 2, and 3 (indicated on the inset). On the right-chromatographic profile of an ethanol extract sample on a ZORBAX Eclipse XDB-C18 column (9.4 × 250 mm). In the middlemean luminescence activity of isolated peaks with C. variopedatus luciferase in RLU (relative light units) (measured in triplicate, expressed as mean ± SD (n = 3) and analyzed using Student's t-test; differences were considered significant at p < 0.05).
Compounds Hitik1 and Hitik2 ( Figure 1) provided indistinguishable mass spectra with the major peaks at m/z 483.2596, 501.2699, and 536.3078 in positive mode. Analysis of the NMR spectra revealed that both compounds contained three identical residues with altered modes of linkages: hexopyranose carbohydrate, glycerol, and a modified polyunsaturated fatty acid. In Hitik1, the fatty acid is linked to the second carbon (2 ) of the glycerol moiety, while for Hitik2, glycerol is substituted at positions 1 and 3 . Analysis of hexopyranose allowed us to assign it unambiguously to β-galactose based on the 13 C chemical shifts (CASPER web server (http://www.casper.organ.su.se/casper/) [11]) and J-couplings. H-1 -H-2 coupling is equal to 7 Hz, which excludes the mannose configuration and suggests the β-configuration of the anomeric carbon; and a small J-coupling value (less than 2 Hz) is observed between the protons H-4 and H-5 , implying the galactose configuration of the hexapyranose. Unlike  Figure 1 and Supplementary Material Tables S1-S3 (Supporting Information).

Synthesis of Bioluminescent Substrate Hitik3
One of the luminescent compounds, named Hitik3, was identified as linoleic acid hydroperoxide at position 9 (9-(E,Z)-HPODE) using NMR and HRMS (high resolution mass-spectrometry) analysis ( Figure 1). To ensure the correct elucidation of the structure of this natural product and for further studies of its possible role in the bioluminescence system of C. variopedatus, it was necessary to synthesize it in sufficient quantities.
There are several well-established approaches to peroxidation of polyunsaturated fatty acids (PUFA): peroxidation with lipoxygenases, singlet oxygen, or atmospheric oxygen [12][13][14]. PUFAs are usually peroxidized at different positions and with divergent configurations of double bonds in the products (Scheme 1). To obtain the target hydroperoxide 9-(E,Z)-HPODE, we tested two of the described approaches: oxidation by singlet oxygen, generated in the presence of methylene blue, and by soybean lipoxygenase.
couplings. H-1′'-H-2′' coupling is equal to 7 Hz, which excludes the mannose configuration and suggests the β-configuration of the anomeric carbon; and a small J-coupling value (less than 2 Hz) is observed between the protons H-4′' and H-5′', implying the galactose configuration of the hexapyranose. Unlike Hitik3, the fatty acid had a length of 16 carbon atoms and contained 3 double bonds at positions 4 (Z), 8 (E), and 10 (Z), with the hydroperoxide group at C-7, resulting in (4Z,8E,10Z)-7-hydroperoxyhexadeca-4,8,10-trienoic acid. The mass of M + H + peak of the compound is equal to 519.2800, which does not correspond to any of the peaks in MS; however, 501.2699 corresponds to M − H2O + H + , 483.2596 corresponds to M -2 × H2O + H + , while 536.3078 may correspond to M + NH4 + . Most likely, compounds are not stable after ionization and immediately convert into ketones instead of the hydroperoxides. Structures of the compounds and chemical shift assignments are shown in Figure 1 and Supplementary Material Tables S1-S3 (Supporting Information).

Synthesis of Bioluminescent Substrate Hitik3
One of the luminescent compounds, named Hitik3, was identified as linoleic acid hydroperoxide at position 9 (9-(E,Z)-HPODE) using NMR and HRMS (high resolution mass-spectrometry) analysis ( Figure 1). To ensure the correct elucidation of the structure of this natural product and for further studies of its possible role in the bioluminescence system of C. variopedatus, it was necessary to synthesize it in sufficient quantities.
There are several well-established approaches to peroxidation of polyunsaturated fatty acids (PUFA): peroxidation with lipoxygenases, singlet oxygen, or atmospheric oxygen [12][13][14]. PUFAs are usually peroxidized at different positions and with divergent configurations of double bonds in the products (Scheme 1). To obtain the target hydroperoxide 9-(E,Z)-HPODE, we tested two of the described approaches: oxidation by singlet oxygen, generated in the presence of methylene blue, and by soybean lipoxygenase. Scheme 1. Isomers of hydroperoxyoctadecadienoic acid (HPODE) formed during oxidation of linoleic acid. Different products are formed during oxidation by singlet oxygen ( 1 O2), atmospheric oxygen (O2) and soybean lipoxygenase (LOX-IB), shown with relative ratio of products [15].
Incubation of linoleic acid with soybean lipoxygenase (LOX-IB) in 50 mM phosphate buffer at pH = 6 allowed a mixture of peroxides with 9-(E,Z)-and 13-(Z,E)-HPODE being the main components and 9-(E,E)-and 13-(E,E)-HPODE, still obtained by oxidation with oxygen,-the minor ones (Scheme 1) [16]. The preparative separation of such mixtures is a challenge due to the insignificant difference in mobility of the components and their instability, which limits the choice of possible additives in the mobile phase and forces the Scheme 1. Isomers of hydroperoxyoctadecadienoic acid (HPODE) formed during oxidation of linoleic acid. Different products are formed during oxidation by singlet oxygen ( 1 O 2 ), atmospheric oxygen (O 2 ) and soybean lipoxygenase (LOX-IB), shown with relative ratio of products [15].
Incubation of linoleic acid with soybean lipoxygenase (LOX-IB) in 50 mM phosphate buffer at pH = 6 allowed a mixture of peroxides with 9-(E,Z)-and 13-(Z,E)-HPODE being the main components and 9-(E,E)-and 13-(E,E)-HPODE, still obtained by oxidation with oxygen,-the minor ones (Scheme 1) [16]. The preparative separation of such mixtures is a challenge due to the insignificant difference in mobility of the components and their instability, which limits the choice of possible additives in the mobile phase and forces the number of purification steps to be minimized. Normal phase chromatography allowed good separation of 9-(E,Z)-from 13-(Z,E)-HPODE; however, it was difficult to purify target 9-(E,Z)-HPODE from other two isomers (9-(E,E)-and 13-(E,E)-), so the sample of 80% purity 9-(E,Z)-HPODE was used for further experiments (Figure 2). A thorough analysis of the 2D NMR spectra obtained for the samples confirmed the structure proposed for Hitik3 product-9-(E,Z)-HPODE ( Figure 3, Supplementary Materials Figure S2, Table S4).
High amounts of PUFA peroxides are usually obtained by oxidation with lipoxygenases as catalysts. However, the application of the method is limited due to the substrate specificity of enzymes; thus, the singlet oxygen oxidation method is preferred. To study the effect of substrate structure on bioluminescence activity, we obtained peroxides of oleic (C18:1, ω-9), linoleic (C18:2, ω-6) and linolenic acids (C18:3, ω-3) by oxidation with methylene blue as a catalyst (Supplementary Materials Figure S14, Supporting Information). As the total amount of peroxide isomers obtained in the resulting mixtures is equal to the number of allyl positions, individual isolation of the compounds from the mixtures was not possible. For further activity tests, PUFA peroxides were used in the mixtures. number of purification steps to be minimized. Normal phase chromatography allowed good separation of 9-(E,Z)-from 13-(Z,E)-HPODE; however, it was difficult to purify target 9-(E,Z)-HPODE from other two isomers (9-(E,E)-and 13-(E,E)-), so the sample of 80% purity 9-(E,Z)-HPODE was used for further experiments (Figure 2). A thorough analysis of the 2D NMR spectra obtained for the samples confirmed the structure proposed for Hitik3 product-9-(E,Z)-HPODE ( Figure 3, Supplementary Materials Figure S2, Table S4).  High amounts of PUFA peroxides are usually obtained by oxidation with lipoxygenases as catalysts. However, the application of the method is limited due to the substrate specificity of enzymes; thus, the singlet oxygen oxidation method is preferred. To study number of purification steps to be minimized. Normal phase chromatography allowed good separation of 9-(E,Z)-from 13-(Z,E)-HPODE; however, it was difficult to purify target 9-(E,Z)-HPODE from other two isomers (9-(E,E)-and 13-(E,E)-), so the sample of 80% purity 9-(E,Z)-HPODE was used for further experiments (Figure 2). A thorough analysis of the 2D NMR spectra obtained for the samples confirmed the structure proposed for Hitik3 product-9-(E,Z)-HPODE (Figure 3, Supplementary Materials Figure S2, Table S4).  High amounts of PUFA peroxides are usually obtained by oxidation with lipoxygenases as catalysts. However, the application of the method is limited due to the substrate specificity of enzymes; thus, the singlet oxygen oxidation method is preferred. To study

Synthesis of Hitik Structural Analogues
Having developed a good reproducible method for the synthesis of linoleic acid peroxides, we contemplated the possibility of using it for the synthesis of Hitik1 and Hitik2 compounds. These natural products contain a peroxide moiety of 4,7,10-hexadecatrienoic acid, which is much less available than linoleic acid and also does not allow the selective introduction of the OOH group. Chaetopterus luciferase, in its turn, appears to be a promiscuous enzyme with greater substrate specificity. For these reasons, we decided to obtain synthetic analogues of Hitik1 and Hitik2 substrates, containing peroxide moi-eties of linoleic acid (Scheme 2) and a peroxide from commercially available phospholipid 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) (Scheme 3).

Synthesis of Hitik Structural Analogues
Having developed a good reproducible method for the synthesis of linoleic acid peroxides, we contemplated the possibility of using it for the synthesis of Hitik1 and Hitik2 compounds. These natural products contain a peroxide moiety of 4,7,10-hexadecatrienoic acid, which is much less available than linoleic acid and also does not allow the selective introduction of the OOH group. Chaetopterus luciferase, in its turn, appears to be a promiscuous enzyme with greater substrate specificity. For these reasons, we decided to obtain synthetic analogues of Hitik1 and Hitik2 substrates, containing peroxide moieties of linoleic acid (Scheme 2) and a peroxide from commercially available phospholipid 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) (Scheme 3). The target galactolipid peroxides 5-8 were synthesized according to known procedures [17][18][19][20][21] using two different synthetic routes (Scheme 2). First one included the synthesis of a conjugate of linoleic acid with galactose moiety 4 followed by peroxidation with 1 O2 in the presence of methylene blue to provide a range of products 5-8 (Scheme 2A). The resulting mixture of peroxides was separated from the side products using normal phase chromatography, but individual compounds could not be obtained. Second pathway was less convergent and implied selective introduction of the peroxide group with lipoxygenase, followed by the protection of hydroperoxide by α-methoxyisopropyl protecting group, conjugation with a carbohydrate moiety 4 and deprotection to allow two target products 5 and 6. PLPC peroxide 11 was obtained using the described procedure [22] (Scheme 3).

Bioluminescent Activity of the Synthesized Substrates
We proceeded to carry out a series of experiments to investigate the bioluminescent activity of the synthesized substrates and mixtures. All experiments were measured while mixing the substrates with partially purified C. variopedatus luciferase and Fe 2+ ions ( Figure  4, Figure S13, Supporting information). The maximum wavelength of bioluminescence emission for 13-HPODE is 460 nm (Figure 4A), which coincides with the data obtained for Chaetopterus mucus: 463 nm [23] or 455 nm [8]-indirectly supporting the hypothesis that the natural Chaetopterus luciferin could have the same active moiety as the Hitik com- The target galactolipid peroxides 5-8 were synthesized according to known procedures [17][18][19][20][21] using two different synthetic routes (Scheme 2). First one included the synthesis of a conjugate of linoleic acid with galactose moiety 4 followed by peroxidation with 1 O 2 in the presence of methylene blue to provide a range of products 5-8 (Scheme 2A). The resulting mixture of peroxides was separated from the side products using normal phase chromatography, but individual compounds could not be obtained. Second pathway was less convergent and implied selective introduction of the peroxide group with lipoxygenase, followed by the protection of hydroperoxide by α-methoxyisopropyl protecting group, conjugation with a carbohydrate moiety 4 and deprotection to allow two target products 5 and 6. PLPC peroxide 11 was obtained using the described procedure [22] (Scheme 3).

Bioluminescent Activity of the Synthesized Substrates
We proceeded to carry out a series of experiments to investigate the bioluminescent activity of the synthesized substrates and mixtures. All experiments were measured while mixing the substrates with partially purified C. variopedatus luciferase and Fe 2+ ions ( Figure 4, Figure S13, Supporting information). The maximum wavelength of bioluminescence emission for 13-HPODE is 460 nm (Figure 4A), which coincides with the data obtained for Chaetopterus mucus: 463 nm [23] or 455 nm [8]-indirectly supporting the hypothesis that the natural Chaetopterus luciferin could have the same active moiety as the Hitik compounds. Bioluminescence kinetics of 13-HPODE demonstrates a characteristic flash profile ( Figure 4B).  The peroxide mixtures from oleic and linolenic acids were almost inactive in the bioluminescence reaction ( Figure 4C, Supplementary Materials Figure S14, Supporting Information). Mixtures of linoleic acid peroxides were less active than 13-HPODE, which is probably due to the presence of 10-and 12-HPODE in the sample. The highest relative total luminescence activity was observed for the substrates, bearing a carbohydrate moiety 5 and 6 ( Figure 4C), which could be explained by their better solubility in aqueous buffers. We also found that compounds with the OOH group at C-9 are more active than their C-13 isomers ( Figure S15, Supporting Information). Interestingly, PLPC peroxide (PLPCOOH) demonstrated almost the same activity as 13-HPODE substrate ( Figure S16, Supporting Information). The results indicate that at least two conjugated bonds in the peroxide are required for activity, and the position of the OOH group in the hydrocarbon . Normalized bioluminescent activity of synthetic substrates. Activity data expressed as the net area under the curve of the luminescence reaction kinetics measured over 3 min (total bioluminescence), normalized by the total bioluminescence of Gal_gly_9-HPODE. Data are measured in triplicate, expressed as mean ± SD (n = 3), and analyzed using Student's t-test; differences were considered significant at p < 0.05. The peroxide mixtures from oleic and linolenic acids were almost inactive in the bioluminescence reaction ( Figure 4C, Supplementary Materials Figure S14, Supporting Information). Mixtures of linoleic acid peroxides were less active than 13-HPODE, which is probably due to the presence of 10-and 12-HPODE in the sample. The highest relative total luminescence activity was observed for the substrates, bearing a carbohydrate moiety 5 and 6 ( Figure 4C), which could be explained by their better solubility in aqueous buffers. We also found that compounds with the OOH group at C-9 are more active than their C-13 isomers ( Figure S15, Supporting Information). Interestingly, PLPC peroxide (PLPCOOH) demonstrated almost the same activity as 13-HPODE substrate ( Figure S16, Supporting Information). The results indicate that at least two conjugated bonds in the peroxide are required for activity, and the position of the OOH group in the hydrocarbon chain is important. In summary, because bioluminescent activity is strongly influenced by changes in the methyl end (ω-3 vs ω-6 acids and the relative position of the peroxide), we suppose that the substrates interact with the luciferase with this part of the molecule.

Mechanism of Chaetopterus Bioluminescence and Bioimaging of Ferroptosis
Usually, the luciferin molecule contains a system of conjugated bonds representing a chromophore, which participates in the process of light emission. In that case, the fluorescence spectrum of the luciferin oxidation product, namely oxyluciferin, coincides with the bioluminescence spectrum [1]. However, for some glowing organisms, such as bacteria, the mollusc Latia neritoides and the worm Diplocardia longa, luciferins and oxyluciferins are small organic molecules that do not contain a system of conjugated bonds. In bacteria, the cofactor, a hydroxylated derivative of flavin mononucleotide, acts as a light emitter in the bioluminescence reaction [24]. For Latia and Diplocardia the light emitters are still unknown. In Siberian earthworms Henlea sp. with the luciferin of yet unreported structure, the proposed light emitter (deazaflavin cofactor) is the molecule that is different from it [25]. Neither partially purified Chaetopterus luciferase nor the studied substrates are fluorescent in the spectral region near Chaetopterus bioluminescence. Thus, the mechanism of light emission in the Chaetopterus bioluminescence reaction remains intriguing and is of certain fundamental and practical interest. The studies of the mechanism should include the isolation of the possible oxidation product, the oxyluciferin, which has not yet been performed.
Ferroptosis, a recently discovered type of programmed cell death, is accompanied by uncontrolled iron-mediated lipid peroxidation [26,27]. Accumulation of Fe 2+ ions and formation of lipid peroxides leading to cell damage is also observed in a number of neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Friedreich's ataxia, and other pathological processes, including amyotrophic lateral sclerosis, periventricular leukomalacia, ischemic stroke, traumatic brain damage, and acute kidney injury. All these health disorders are presumably associated with the launch of the ferroptosis cascade [28]. In order to investigate the mechanisms of ferroptosis in living cells, certain bioimaging strategies, including fluorescent probes for measurement of Fe 2+ , Fe 3+ ions, ROS (reactive oxygen species), etc., were developed [29,30]. The main disadvantage of these strategies is that they use indirect approaches and could also indicate other pathological processes, such as lipid peroxidation or oxidative stress, but not exactly ferroptosis. In the meantime, Chaetopterus luciferase, which utilizes both PUFA or phospholipid hydroperoxides and ferrous ions, might overcome these limitations and become a useful tool for existing bioimaging methods, when the mechanism of luminescence is thoroughly studied and the active recombinant luciferase is available.
In conclusion, we isolated and identified the compounds that demonstrate bioluminescence activity with Chaetopterus luciferase. The structures of dienoic acids peroxides and their galactose conjugates were confirmed by using NMR and total synthesis. We also synthesized a number of their structural analogues and showed that Chaetopterus luciferase has broad substrate specificity and could catalyze the bioluminescence reaction for peroxides of certain polyunsaturated fatty acids and their derivatives, as well as phospholipid peroxides, in the presence of ferrous ions. However, the mechanism of light emission in Chaetopterus bioluminescence reaction is still unknown. Further studies of Chaetopterus luciferase are of great interest as they may allow the development of new tools for the bioimaging of ferroptosis in living cells.

Chaetopterus Variopedatus Biomass and Luciferase
Chaetopterus variopedatus was collected at two locations: São Sebastião Strait of the coast of Brazil and Trinity bay in the Possiet Gulf of Japan Sea. The worms were pulled out of their tubes and immediately frozen in liquid nitrogen. The frozen polychaetes were shipped on dry ice and stored at -70 • C.

Hitik1, Hitik2, Hitik3 Purification Procedure
Algae of the species Chaetomorpha linum from a private marine aquarium were used. An amount of 100 g (wet biomass) of algae C. linum were removed from the aquarium and rinsed with distilled water. All further procedures were carried out at 4 • C. The cell fluid from the algae biomass was squeezed out using a manual juicer in order to avoid heating of the resulting extract. Ethyl alcohol, cooled to −20 • C, was added to the obtained extract to a final concentration of 60%. Then, the extract was centrifuged at 25,000× g 20 min in an Avanti ® J-E centrifuge (Beckman Coulter, Brea, CA, USA). The precipitate was discarded; supernatant was diluted twofold with 10 mM phosphate buffer, pH 9.0. The resulting extract was passed through a 30 × 100 mm Cellulose DEAE 32 column with BioLogic Duo-Flow chromatography system (Bio-Rad Laboratories, Hercules, CA, USA). The filtrate was applied to a 20 × 100 mm column containing the Diasorb-60-C16T sorbent (Biohimmak, Moscow, Russia). The column was equilibrated and washed with a solution of 50% aqueous ethanol. The substance was eluted from the sorbent with 96% ethanol isocratically. Luminescent active fractions were collected, dried on a rotary evaporator, diluted in 400 µL of 96% ethanol, and purified using HPLC. A semi-preparative column (9.4 × 250 mm), ZORBAX Eclipse XDB-C18 (Agilent Technologies, Santa Clara, CA, USA), connected to an Agilent 1260 Infinity LC chromatography system (Agilent Technologies, Santa Clara, CA, USA) was used. Elution was performed using a gradient elution program: solvent A-0.1% formic acid and solvent B-acetonitrile. The standard gradient program was 5-40% B over 25 min, with a flow rate of 3 mL/min. Absorption was monitored at 210, 230, 250, 270, 290, 310, 330, and 360 nm. Fractions, which demonstrated bioluminescence activity and corresponded to peaks 1-3, were collected separately ( Figure 1). As a result, three active samples were obtained. The samples were rechromatographed separately on the same column. The resulting active fractions of the peaks were dried on an Eppendorf concentrator 5301rotary evaporator (Eppendorf, Hamburg, Germany) at r. t. and diluted in 100 µL of 96% ethanol, then chromatographed on gel filtration chromatography using Superdex Peptide 10/300 GL column (Cytiva, Marlborough, MA, USA) in a mixture of 0.1% formic acid and 50% acetonitrile. Fractions, corresponding to peaks 1, 2, and 3, named Hitik1 (2.6 absorbance units), Hitik2 (2.9 absorbance units), and Hitik3 (0.15 absorbance units), respectively, which exhibited bioluminescence activity when mixed with partially purified luciferase C. variopedatus [9] and ferrous ions Fe 2+ , were collected and dried on a rotary evaporator.

Methylene Blue Catalyzed Photo-Oxidation of PUFA or Their Derivatives
PUFA and their derivatives (0.1 mmol) were dissolved in 1 mL of methanol and the resulting solution was placed in an ampoule. A solution of methylene blue in methanol was added at a concentration of 0.1 mg/mL (0.13 mmol). A 150 W incandescent lamp (Nova II) was placed at a distance of 15-20 cm in front of the ampoule, and oxygen was bubbled through the reaction mixture for 4-6 h until TLC (thin layer chromatography) (CH 2 Cl 2 : Isopropanol, 95:5) showed complete conversion of the starting PUFA and their derivatives into more polar products. Products were further purified using RP-HPLC on the PuriFlash 5.250 system with UV-detector (Interchim, Montluçon, France) with 150 × 10 mm Luna 10 um C18(2) column (Phenomenex, Torrance, CA, USA) eluted with acetonitrile/water linear gradient-50-100% acetonitrile, flow rate = 5 mL/min, λ = 235 nm.

Bioluminescence Spectrum of 13-HPODE
A 50 µL aliquot of the partially purified Chaetopterus enzyme [9] was incubated for 10 min in the presence of 2 mM 13-HPODE in 200 µL of 50 mM phosphate buffer (pH 8.0). Then, the sample was placed in a cuvette within a Shimadzu-UV-Mini-1240-UV-VIS Spectrophotometer (Shimadzu, Kyoto, Japan). A solution of FeSO 4 was continuously pumped into the cuvette at a rate of 0.25 mM / min, and the chemiluminescent visible light spectrum was measured from the moment pumping began for up to 5 min or until the luminescent reaction was below the level of detection for the spectrophotometer.

Bioluminescence Activity Assay
Reactions were monitored with a custom made luminometer BLM (Oberon-K, Krasnoyarsk, Russia). For each measurement 100 µL of reaction mixture (phosphate-buffered saline (pH 7.4)), 1 µL of luciferase fraction, 1 µL of 3 mM peroxide methanolic solution, and 1 µL 10 mM FeSO 4 solution) were used. Measurements were corrected for background luminescence of PUFA peroxides in the presence of Fe 2+ ions. All experiments were performed in triplicate. The data are expressed as mean ± SD (n = 3) and analyzed using Student's t-test. Differences were considered significant at p < 0.05.

Other Methods, Synthetic Procedures, and NMR Spectra
Details of the other materials and methods used in this study, including procedures to obtain synthetic compounds and their characterization using MS and NMR (Figures S3-S12) are provided in Supporting Information.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available in the Supporting Information file. Additional data are available from the corresponding author on reasonable request.